Dopamine's Regulation of Insulin Secretion Through a Receptor Complex

Dopamine's Regulation of Insulin Secretion Through a Receptor Complex ...

Dopamine (DA) has shown that insulin production is important in the body. In response to the glucosea process called glucose-stimulated insulin secretion (GSIS), insulin is negatively regulated, thus making transient changes in the body''s insulin levels. However, this mechanism has been unknown until today.

A group led by scientists from the Tokyo Institute of Technology (Tokyo Tech) claims it has discovered the exact mechanism through which DA regulates insulin secretions. Using total internal reflection fluorescence microscopy, they discovered that DA receptors D1 and D2 (proteins on cells that DA can bind to) act in tandem to achieve the transient regulation of insulin. Dopamine, according to their findings, regulates insulin secretion by activation of the D1-D2 receptor heteromer, in

We also found that the dopamine-mediated inhibition of insulin secretion was reduced by D1 receptor antagonists. This further revealed that overexpression of only D2 receptors on beta cells exerted an inhibitory and toxic effect, thus abolishing insulin secretion in beta-cells. This gave us a clue to the mechanism of down-regulation, according to Tokyo Techs.

The researchers used proximity ligation and Western blot experiments to examine the receptors further. D1 and D2 were bound to each other to form a complex (heteromer) when activated by DA. D1 and D2 were transiently inhibited insulin secretion. They also discovered that when D1 and D2 were co-expressed on beta cells, the cells were able to avoid the harmful effects of D2 overexpression.

According to Kume, from these conclusions, D1 modulates D2 signaling to protect beta-cells from the harmful effects of DA. This study greatly improves our understanding of DA signaling in diabetics.

Understanding the effect of DA signaling in the regulation of insulin secretion is certain to offer new therapeutic goals for diabetes prevention, treatment, and treatment.

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