Target enrichment techniques for next-generation sequencing (NGS) provide solutions to focus on and sequence a small portion of the human genome. This reduces sequencing and analysis costs for laboratorys that study genetic diseases. These savings typically range from around 100 to 100,000 fold depending on the application and the amount of the genome being targeted.
Hybridization capture techniques are considered high-performance approaches that require lengthy steps and an overnight hybridization incubation to ensure excellent performance. On the other hand, amplicon technologies are considered fast and easy to use, but generally do not have the same level of performance as capture technologies. Primer Extension Target Enrichment (PETE) is a newly developed technology that combines DNA polymerase extension reactions to provide a high-performance target enrichment solution that is easy to use and can be completed in a single day.
Brian Godwin, the director of reagent and assay development at Roche Sequencing, gives us an overview of each of the three target enrichment technologies along with a description of common target enrichment metrics.
Do you want to learn more?
- KAPA HyperPETE Brochure