Target enrichment methods for next-generation sequencing (NGS) provide solutions to focus on and sequence a small portion of the human genome. This reduces laboratory sequencing and analysis expenses. These savings generally range from around 100 to 100,000 fold depending on the type of genome being targeted.
Hybridization detection techniques are considered high-performance applications that require long-term surgery and an overnight hybridization incubation. On the other hand, amplicon technologies are considered fast and easy to obtain, although they may not achieve the same level of performance as capture techniques. Primer Extension Target Enrichment (PETE) is a newly developed technology that combines DNA polymerase extensions into a single-day capsule.
Brian Godwin, the director of Roche Sequencing''s reagent and assay development, gives us an overview of how each of the three target enrichment technologies work along. Godwin also gives us a description of common target enrichment performance metrics.
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- KAPA HyperPETE Brochure