A novel technique converts human pluripotent stem cells to an 8-cell embryo-like stage

A novel technique converts human pluripotent stem cells to an 8-cell embryo-like stage ...

Scientists from BGI-Research, the Chinese Academy of Sciences, and a group of partners wrote a study inNature, which announced the discovery of a transgene-free, rapid, and controllable approach to convert pluripotent stem-cells intobona fide8-cell totipotent embryo-like cells, paving the way for advancements in organ regeneration and synthetic biology.

BGI''s advanced single-cell sequencing techniques were used by BGI researchers to convert pluripotent stem cells, or an adult version of early embryonic cells, into a more juvenile version of cells that are capable of human zygotic genome activation.

These cells might be used in regenerative medicine in the future to regenerate human organs that have become diseased and reduce the world''s dependence on organ donation. They are also effective in reducing human embryonic development, while also assisting in early developmental health issues.

The first time this research has been undertaken for the first time. This study will help scientists understand early human embryonic development. Crucially, the researchers were able to demonstrate that the converted cells might be used to create placental cellsin vivo.

According to the authors of the paper, Totipotent 8-cell stage embryo-like cells can not only differentiate into placental tissue but also develop into more mature organs, bringing good news to millions of patients who have no organ transplants in the world.

"This breakthrough is a promising example of the combination of regenerative medicine and single-cell sequencing technology," said Dr. Liu Longqi of BGI-Research, another author. "Through large-scale single-cell multi-omics profiling, the efficient and precise identification of cells or tissues obtained in vitro or in vivo will greatly accelerate researches on regenerative medicine.

Cells at this early stage of development may be described as totipotent, meaning they have the potential to create all kinds of early embryonic cells, which in turn transform the tissues and organs necessary for development. The research concludes in a previous study with pluripotent stem cells at the blastocyst stage, allowing cells to create a wider spectrum of cellular components and tissues.

Advances in single-cell sequencing techniques are facilitating the breakthrough, which means BGI Group is a world leader. Its single-cell library sequencing platform, combined with its sequencing technology, makes for extensive and multi-dimensional single-cell analysis at an affordable cost.

The international team behind the research took pluripotent stem cells and treated them with a chemical cocktail to create the 8-cell embryo-like cells. Among other experiments, these cells were then sorted and injected into a mouse to undergo further research, and then analysed using BGIs single-cell genomic analysis. This innovative technique helped scientists to identify and isolate the target 8-cell embryo-like cells and demonstrate their totipotent ability to produce the cells involved in generating the placentain vivo.

The advancements being made by the researchers might eventually make individual organ regeneration a reality. Typically, the only method available for organ transplanteers is finding a matching donor. Transplants may fail if the donor serotype is too different from the recipient. A separate technique is also in its infancy.

This achievement provides a newin vitroresearch system for basic research on early embryonic development, which assists us understand the relationship between early embryonic development and disease occurrence, as well as a resource for research and treatment of birth defects and various developmental disorders.

The Guangzhou Institutes of Biomedicine and Health of the Chinese Academy of Sciences, BGI-Research, the University of Cambridge, Jilin University in Changqun, and the University of Rajshahi from Bangladesh are some of the research institutions involved in the study. Before the ethical clearance was obtained, the study was approved.

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